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DEPARTMENT OF BIOTECHNOLOGY In the Immunology and Molecular Biology Laboratory of Dept. of Biotechnology the following experiments in the frontier areas are being carried out by the M.Sc., Biotechnology Students • Polymerase Chain Reaction / RTPCR • RAPD / RFLP • AGE • SDS PAGE • Southern / Western Blotting • Immunoelectrophoresis • Rocket Electrophoresis • Biotinylation • Complement Assay • DNA Fragmentation Assay • ELISA • ROS / RON Animal Cell Culture Laboratory of Department of Biotechnology with CO2 Incubator (Sanyo, Japan) facility is being involved in the following • Monolayer production • Primary Cell Culture • Secondary Cell Culture • Organ Culture by agar Plate Method • Lymphocyte Culture The major facilities of the Instrumentation Lab includes • PCR ( M.J. Research , USA) • Gel Documentation Unit ( Vilber Lourmate, France)) • ELISA Reader ( Biorad , USA) • Nucleic Acid Sequencer ( Genei) • Individual Electrophoretic Units • Fermentor Vessel 2L (B.Braun , Germany) • Western Blotting Unit • Plant Tissue Culture Racks with Accessories • Laminar Air Flow Hood ( Multiple numbers) • Cold Storage - 20°C ( Blue Star ) - 70°C( Blue Star ) Cold Chamber ( Blue Star ) The Research Division of Department of Biotechnology is being involved in the • Screening of Phyto immunostimulants for their immunological responses • Isolation and purification of bioactive principles • Synthesis of biopolymers • Evaluation of Microbial organisms as the potential tool in bioremediation
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